4PRQ
CRYSTAL STRUCTURE OF HEN EGG-WHITE LYSOZYME IN COMPLEX WITH SCLX4 AT 1.72 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-22 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.00792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.010, 81.749, 72.100 |
| Unit cell angles | 90.00, 105.37, 90.00 |
Refinement procedure
| Resolution | 42.470 - 1.720 |
| R-factor | 0.1881 |
| Rwork | 0.186 |
| R-free | 0.22197 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z12 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.141 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.470 | 1.820 |
| High resolution limit [Å] | 1.720 | 1.720 |
| Rmerge | 0.089 | 0.694 |
| Number of reflections | 51142 | |
| <I/σ(I)> | 9.4 | 1.8 |
| Completeness [%] | 98.6 | 94.9 |
| Redundancy | 3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293.15 | 24 % PEG 8000, 100 MM MAGNESIUM CHLORIDE, 50 MM SODIUM CHLORIDE, 50 MM SODIUM CACODYLATE, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K |
