4PRO
ALPHA-LYTIC PROTEASE COMPLEXED WITH PRO REGION
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-18 |
| Detector | RIGAKU |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.800, 61.900, 72.600 |
| Unit cell angles | 109.40, 99.20, 102.40 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pro |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.854) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.200 | 2.470 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.071 | 0.371 |
| Total number of observations | 104427 * | |
| Number of reflections | 31304 * | |
| <I/σ(I)> | 17.5 | 3.4 |
| Completeness [%] | 95.9 | 93.9 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 7 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9 (mg/ml) | |
| 10 | 1 | reservoir | lithium sulfate | 0.8 (M) | bufferA |
| 11 | 1 | reservoir | glycerol | 5 (%) | bufferA |
| 12 | 1 | drop | bufferA | 0.45 times | |
| 2 | 1 | drop | lithium sulfate | 0.34 (M) | |
| 3 | 1 | drop | glycerol | 2.3 (%) | |
| 4 | 1 | drop | dithiothreitol | 2 (mM) | |
| 5 | 1 | drop | MES | 2.7 (mM) | |
| 6 | 1 | reservoir | citric acid | 15 (mM) | bufferA |
| 7 | 1 | reservoir | phosphoric acid | 30 (mM) | bufferA |
| 8 | 1 | reservoir | orthoboric acid | 26 (mM) | bufferA |
| 9 | 1 | reservoir | sodium hydroxide | 0.155 (M) | bufferA |
