4PRI
Crystal structure of TK3 TCR-HLA-B*35:08-HPVG complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.984 |
Spacegroup name | P 1 |
Unit cell lengths | 44.580, 62.380, 100.460 |
Unit cell angles | 98.17, 94.59, 109.12 |
Refinement procedure
Resolution | 41.700 - 2.400 |
R-factor | 0.213 |
Rwork | 0.213 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vm7 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.700 | 2.500 | |
High resolution limit [Å] | 2.400 | 10.000 | 2.400 |
Rmerge | 0.070 | 0.018 | 0.323 |
Number of reflections | 73467 | 1019 | 8684 |
<I/σ(I)> | 10.77 | 34.91 | 2.69 |
Completeness [%] | 93.6 | 93.1 | 96.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 298 | 18% PEG 3350, 0.2M LiSO4 and 0.1M Na-Citrate pH 5.6, vapor diffusion, temperature 298K |