4PR8
URATE OXIDASE AZIDE URIC ACID TERNARY complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.938 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 78.577, 95.155, 104.823 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.000 - 1.160 |
R-factor | 0.184 |
Rwork | 0.184 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4fsk |
RMSD bond length | 0.026 |
RMSD bond angle | 0.070 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.000 | 10.000 | 1.280 |
High resolution limit [Å] | 1.158 | 1.280 | 1.158 |
Rmerge | 0.055 | 0.272 | |
Number of reflections | 65154 | ||
<I/σ(I)> | 64.4 | 2.1 | |
Completeness [%] | 80.8 | 97.15 | 34.2 |
Redundancy | 2.5 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH METHOD | 8.5 | 291 | URATE OXIDASE COMPLEXED WITH 8-NITROXANTHINE (RED CRYSTALS-3LD4) SOAKED IN URIC ACID SOLUTION. AFTER LIGAND EXCHANGE, (COLORLESS CRYSTALS) THE CRYSTALS WERE INCUBATED WITH SODIUM AZIDE 0.3M, 18% PEG8000, TRIS BUFFER PH=8.5 FOR 1/2 H., BATCH METHOD, temperature 291K |