4PR5
Crystal structure of a HLA-B*35:01-HPVG-D5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-12 |
Detector | ADSC-Q210 |
Wavelength(s) | 0.984 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.640, 81.230, 109.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.286 - 1.800 |
R-factor | 0.1806 |
Rwork | 0.179 |
R-free | 0.21320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb entry 2FYY |
RMSD bond length | 0.010 |
RMSD bond angle | 1.097 |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 | |
High resolution limit [Å] | 1.800 | 10.000 | 1.800 |
Rmerge | 0.066 | 0.017 | 0.447 |
Number of reflections | 42487 | 285 | 6249 |
<I/σ(I)> | 26.2 | 86.1 | 4.76 |
Completeness [%] | 100.0 | 96 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.6 | 298 | 16% PEG 4000, 0.2M ammonium acetate and 0.1M Na-Citrate pH 5.6, vapor diffusion, temperature 298K |