4PQP

Crystal structure of human SNX14 PX domain in space group P43212

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2013
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 43 21 2
Unit cell lengths	121.670, 121.670, 82.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.948 - 3.000
R-factor	0.2368
Rwork	0.234
R-free	0.28560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.452
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.200
High resolution limit [Å]	3.000	3.000
Rmerge	0.140
Number of reflections	12933
Completeness [%]	100.0
Redundancy	27.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	20% PEG4000, 0.1 M sodium citrate tribasic, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K