4PQL

N-Terminal domain of DNA binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 2 21 21
Unit cell lengths	50.048, 68.968, 86.145
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.275 - 2.444
R-factor	0.2088
Rwork	0.203
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.101
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.480
High resolution limit [Å]	2.440	6.620	2.440
Rmerge	0.061	0.029	0.319
Number of reflections	10776
<I/σ(I)>	12
Completeness [%]	93.2	90	73.2
Redundancy	3.7	3.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1 M Sodium citrate tribasic and 100 mM Sodium cacodylate/ Hydrochloric acid, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K