4PQH
Crystal structure of glutathione transferase lambda1 from Populus trichocarpa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 |
| Unit cell lengths | 33.009, 45.835, 66.739 |
| Unit cell angles | 91.53, 102.43, 96.26 |
Refinement procedure
| Resolution | 45.500 - 1.400 |
| R-factor | 0.1487 |
| Rwork | 0.148 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pqi |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.025 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.590 |
| Number of reflections | 71143 | |
| <I/σ(I)> | 16.3 | 2.4 |
| Completeness [%] | 95.2 | 93.1 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 291 | 30% (w/v) PEG4000, 200 mM ammonium acetate and 100 mM sodium acetate, pH 4.6, VAPOR DIFFUSION, temperature 291K |
