4PPL
Crystal structure of eCGP123 H193Q variant at pH 7.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.560, 83.390, 74.650 |
| Unit cell angles | 90.00, 102.19, 90.00 |
Refinement procedure
| Resolution | 46.210 - 2.200 |
| R-factor | 0.1947 |
| Rwork | 0.190 |
| R-free | 0.28690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ECGP123 WITHOUT CHROMOPHORE WATERS AND SUBSTITUTION |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.210 |
| High resolution limit [Å] | 2.200 |
| Completeness [%] | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 293 | 21% PEG 3350, 0.2M MgCl2, 0.1M BTP ph 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
