4PPJ
Crystal structure of Phanta, a weakly fluorescent photochromic GFP-like protein. ON state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.815, 79.370, 71.001 |
| Unit cell angles | 90.00, 102.40, 90.00 |
Refinement procedure
| Resolution | 30.680 - 2.300 |
| R-factor | 0.2001 |
| Rwork | 0.197 |
| R-free | 0.26270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | ECGP123 WITHOUT CHROMOPHORE WATERS AND SUBSTITUTIONS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.160 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 79.370 |
| High resolution limit [Å] | 2.300 |
| Completeness [%] | 98.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 293 | 20% PEG3350, 0.15M MgCl2, 0.1M Tris-HCl pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
