4PPH
Crystal structure of conglutin gamma, a unique basic 7S globulin from lupine seeds
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-07 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 31 |
| Unit cell lengths | 121.990, 121.990, 188.490 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.764 - 2.009 |
| R-factor | 0.1457 |
| Rwork | 0.145 |
| R-free | 0.17400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3aup chain A |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.425 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.760 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.068 | 0.755 |
| Number of reflections | 208256 | |
| <I/σ(I)> | 13.22 | 1.98 |
| Completeness [%] | 99.7 | 99.3 |
| Redundancy | 3.86 | 3.86 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 7% PEG 6000, 0.1M HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
