4PPH
Crystal structure of conglutin gamma, a unique basic 7S globulin from lupine seeds
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.2 |
Synchrotron site | BESSY |
Beamline | 14.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-07 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 31 |
Unit cell lengths | 121.990, 121.990, 188.490 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.764 - 2.009 |
R-factor | 0.1457 |
Rwork | 0.145 |
R-free | 0.17400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3aup chain A |
RMSD bond length | 0.011 |
RMSD bond angle | 1.425 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.3_1479)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.760 | 2.120 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.068 | 0.755 |
Number of reflections | 208256 | |
<I/σ(I)> | 13.22 | 1.98 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 3.86 | 3.86 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 292 | 7% PEG 6000, 0.1M HEPES pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |