4POZ
Fab fragment of Der p 1 specific antibody 10B9
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-11 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 67.020, 121.829, 55.041 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.750 |
R-factor | 0.17065 |
Rwork | 0.169 |
R-free | 0.20587 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rvt |
RMSD bond length | 0.019 |
RMSD bond angle | 1.882 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.069 | 0.633 |
Number of reflections | 45773 | |
<I/σ(I)> | 27 | 2.2 |
Completeness [%] | 99.0 | 96.9 |
Redundancy | 5.4 | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100 mM sodium citrate, 15% w/v PEG 6000, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |