4POG
MCM-ssDNA co-crystal structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-30 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.277, 113.398, 196.855 |
| Unit cell angles | 90.00, 101.35, 90.00 |
Refinement procedure
| Resolution | 48.510 - 3.203 |
| R-factor | 0.25877 |
| Rwork | 0.257 |
| R-free | 0.29399 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pof |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.164 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.310 |
| High resolution limit [Å] | 3.203 | 3.203 |
| Rmerge | 0.109 | 0.786 |
| Number of reflections | 66920 | |
| <I/σ(I)> | 13.4 | 1.64 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 50 mM MES, pH 6.0, 10 mM magnesium acetate, 28.5% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
