4POF
PfMCM N-terminal domain without DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 122.849, 103.064, 122.435 |
| Unit cell angles | 90.00, 119.85, 90.00 |
Refinement procedure
| Resolution | 47.330 - 2.648 |
| R-factor | 0.25978 |
| Rwork | 0.259 |
| R-free | 0.26992 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ltl |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.361 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.740 |
| High resolution limit [Å] | 2.648 | 2.648 |
| Rmerge | 0.100 | 0.569 |
| Number of reflections | 76233 | |
| <I/σ(I)> | 16.3 | 2.26 |
| Completeness [%] | 98.8 | 98.2 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.2 M sodium malonate, pH 7.0, 20% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291.0K |
