4POE
Urate oxidase co-crystallized with uric acid and azide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-29 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 79.524, 95.240, 104.577 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.000 - 1.070 |
R-factor | 0.185 |
Rwork | 0.185 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 3l8w |
RMSD bond length | 0.030 |
RMSD bond angle | 0.076 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.600 | 1.120 |
High resolution limit [Å] | 1.050 | 1.050 |
Rmerge | 0.068 | 0.325 |
Number of reflections | 148909 | |
<I/σ(I)> | 2 | |
Completeness [%] | 99.3 | 54.8 |
Redundancy | 4.4 | 3.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 291 | MIXING TWO SOLUTIONS A AND B: SOLUTION A: PROTEIN AT 20MG/ML IN 50MM TRIS-ACETATE BUFFER, + 0.3M SODIUM AZIDE SATURATED BY URIC ACID (IN PRESENCE OF THE SOLID), PH 8.5 SOLUTION B: PEG-8000 18% IN TRIS BUFFER, BATCH METHOD, temperature 291K |