4PO1
Mycobacterium tuberculosis RecA glycerol bound room temperature structure IIC-RT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2012-05-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54179 |
Spacegroup name | P 61 |
Unit cell lengths | 108.340, 108.340, 72.540 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 21.150 - 3.400 |
R-factor | 0.17113 |
Rwork | 0.165 |
R-free | 0.22868 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g19 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.076 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.390 | 3.580 |
High resolution limit [Å] | 3.400 | 3.400 |
Rmerge | 0.337 | 0.872 |
Number of reflections | 6799 | |
<I/σ(I)> | 7.6 | 2.6 |
Redundancy | 6 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 298 | 15% PEG3350, 10% PEG5000 MME, 0.2M AMMONIUM ACETATE, 0.1M SODIUM CITRATE , pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |