4PNY
Crystal structure of Staphylococcal nuclease variant Delta+PHS I72H at cryogenic temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2013-06-22 |
Detector | BRUKER APEX |
Wavelength(s) | 1.54 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.172, 60.573, 37.442 |
Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
Resolution | 31.800 - 1.680 |
R-factor | 0.1975 |
Rwork | 0.195 |
R-free | 0.24170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3bdc |
RMSD bond length | 0.016 |
RMSD bond angle | 1.904 |
Data reduction software | SAINT |
Data scaling software | SAINT |
Phasing software | PHASER (2.5.1) |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.680 | 1.680 |
Rmerge | 0.030 | 0.209 |
Number of reflections | 15921 | |
<I/σ(I)> | 20.59 | 4.35 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8 | 5.21 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20% MPD, 25 mM potassium phosphate, calcium chloride, pdTp, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |