4PNO

Escherichia coli Hfq-RNA complex at 0.97 A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.885611
Spacegroup name	P 6
Unit cell lengths	61.110, 61.110, 27.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.555 - 0.970
R-factor	0.1437
Rwork	0.142
R-free	0.16390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hk9
RMSD bond length	0.018
RMSD bond angle	1.866
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.920	1.005
High resolution limit [Å]	0.970	0.970
Rmerge	0.033
Number of reflections	68880
<I/σ(I)>	26
Completeness [%]	100.0	100
Redundancy	10.2	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	12% (w/v) PEG 3350; 0.1M Hepes/NaOH, pH 8.0; 0.25M KSCN