4PNH
Crystal structure of D,D-heptose 1,7-bisphosphate phosphatase from Burkholderia Thailandensis
Replaces: 4JYRExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-07-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 245.904, 245.904, 41.398 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.010 - 2.660 |
| R-factor | 0.2162 |
| Rwork | 0.214 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.173 |
| Refinement software | PHENIX ((phenix.refine: dev_1420)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.860 | 2.710 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.043 | 0.213 |
| Number of reflections | 70758 | |
| <I/σ(I)> | 16.8 | 3.1 |
| Completeness [%] | 87.9 | 86.1 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 296 | 1.3M AMMONIUM SULFATE, 0.1M SODIUM ACETATE PH 4.8 |
