4PN1

Structure of S. pombe Pct1 RNA triphosphatase in complex with the Spt5 CTD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 2
Unit cell lengths	95.603, 251.542, 75.397
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.362 - 2.803
R-factor	0.1942
Rwork	0.191
R-free	0.24770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pn0
RMSD bond length	0.003
RMSD bond angle	0.681
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.113	0.430
Number of reflections	49568
<I/σ(I)>	13.8	2.6
Completeness [%]	99.1	97.7
Redundancy	4.9	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	3.0 M sodium formate cryo-protected in 3.2 M sodium formate and 20% (v/v) ethylene glycol