4PN0

Structure of S. pombe Pct1 RNA triphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-06-22
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	66.340, 93.660, 123.980
Unit cell angles	90.00, 104.10, 90.00

Refinement procedure

Resolution	21.476 - 2.600
R-factor	0.2011
Rwork	0.199
R-free	0.24520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d8i
RMSD bond length	0.002
RMSD bond angle	0.643
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.091	0.457
Number of reflections	45261
<I/σ(I)>	11.9	2.2
Completeness [%]	91.2	78.8
Redundancy	2.4	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2.8 M NaCl, 10% (w/v) PEG6000