4PMX

Crystal structure of GH10 endo-b-1,4-xylanase (XynB) from Xanthomonas axonopodis pv citri in the native form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-05
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.03
Spacegroup name	P 21 21 21
Unit cell lengths	49.463, 71.834, 83.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.950 - 1.304
R-factor	0.1333
Rwork	0.132
R-free	0.17430
Structure solution method	SAD
RMSD bond length	0.023
RMSD bond angle	2.020
Data reduction software	HKL-2000
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.320
High resolution limit [Å]	1.300	3.530	1.300
Rmerge	0.040	0.016	0.561
Total number of observations	200829
Number of reflections	69082
<I/σ(I)>	13.5
Completeness [%]	94.4	99	95.5
Redundancy	2.9	3.4	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Polyethylene glycol 8,000