4PMV
Crystal structure of a novel reducing-end xylose-releasing exo-oligoxylanase (XynA) belonging to GH10 family (space group P43212)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.45 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 78.819, 78.819, 303.969 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.140 - 3.001 |
| R-factor | 0.2269 |
| Rwork | 0.226 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pmu |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.922 |
| Data reduction software | HKL-2000 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 8.130 | 3.000 |
| Rmerge | 0.160 | 0.045 | 0.704 |
| Total number of observations | 205646 | ||
| Number of reflections | 19710 | ||
| <I/σ(I)> | 5.3 | ||
| Completeness [%] | 97.4 | 100 | 99 |
| Redundancy | 10.4 | 10.9 | 11.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | Polyethylene glycol 6000 |
