4PMH
The structure of rice weevil pectin methyl esterase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | AREA DETECTOR |
| Collection date | 2000-05-26 |
| Detector | CUSTOM-MADE |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.429, 73.939, 204.897 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 1.790 |
| R-factor | 0.1756 |
| Rwork | 0.173 |
| R-free | 0.22580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.290 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 99.000 | 99.000 | 1.850 |
| High resolution limit [Å] | 1.790 | 3.850 | 1.790 |
| Rmerge | 0.085 | 0.066 | 0.296 |
| Total number of observations | 95040 | ||
| Number of reflections | 23540 | ||
| <I/σ(I)> | 5.8 | ||
| Completeness [%] | 85.6 | 98.1 | 50.7 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 298 | unknown |
