4PM8

Crystal structure of CTX-M-14 S70G:S237A beta-lactamase at 1.17 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	80
Detector technology	CCD
Collection date	2014-04-04
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97
Spacegroup name	P 21 21 21
Unit cell lengths	41.500, 62.190, 86.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.245 - 1.169
R-factor	0.1267
Rwork	0.126
R-free	0.14280
RMSD bond length	0.005
RMSD bond angle	1.115
Data reduction software	iMOSFLM (1.0.7)
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.300
High resolution limit [Å]	1.169
Number of reflections	76012
<I/σ(I)>	17.9
Completeness [%]	99.9
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	Ammonium sulfate, PEG 4000