4PLZ
Crystal structure of Plasmodium falciparum lactate dehydrogenase mutant W107fA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2012-12-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115866 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.530, 86.010, 91.440 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.720 - 1.050 |
| R-factor | 0.1304 |
| Rwork | 0.130 |
| R-free | 0.14570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t2d |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.588 |
| Data reduction software | XDS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.080 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmerge | 0.029 | 0.420 |
| Number of reflections | 284505 | |
| <I/σ(I)> | 18.9 | 1.81 |
| Completeness [%] | 99.3 | 97.1 |
| Redundancy | 2.9 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 20mg/mL protein with 1.2mM oxamate/2mM NADH; 22% PEG-1000 |
