4PLX

Crystal structure of the triple-helical stability element at the 3' end of MALAT1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-12-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	162.820, 162.820, 65.930
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.200 - 3.100
R-factor	0.222
Rwork	0.220
R-free	0.25500
RMSD bond length	0.010
RMSD bond angle	1.026
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.180
High resolution limit [Å]	3.100	3.100
Number of reflections	17565
<I/σ(I)>	17.2	1.28
Completeness [%]	99.7	99.8
Redundancy	11.2	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Folded RNA was combined with an equal volume of the reservoir solution (50 mM sodium cacodylate pH 6.5, 18 mM magnesium chloride, 2.5 mM spermine and 9% isopropanol)
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Folded RNA was combined with an equal volume of the reservoir solution (50 mM sodium cacodylate pH 6.5, 18 mM magnesium chloride, 2.5 mM spermine and 9% isopropanol)
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	Folded RNA was combined with an equal volume of the reservoir solution (50 mM sodium cacodylate pH 6.5, 18 mM magnesium chloride, 2.5 mM spermine and 9% isopropanol)