4PLR
Crystal Structures of Designed Armadillo Repeat Proteins: Implications of Construct Design and Crystallization Conditions on Overall Structure.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-27 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 41 |
| Unit cell lengths | 116.054, 116.054, 86.932 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.563 - 2.100 |
| R-factor | 0.1761 |
| Rwork | 0.172 |
| R-free | 0.24770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4db9 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 82.062 | 44.563 | 2.210 |
| High resolution limit [Å] | 2.100 | 6.640 | 2.100 |
| Rmerge | 0.061 | 0.661 | |
| Rmeas | 0.129 | ||
| Rpim | 0.051 | 0.027 | 0.288 |
| Total number of observations | 212948 | 6316 | 30917 |
| Number of reflections | 33688 | ||
| <I/σ(I)> | 8 | 16.6 | 2.6 |
| Completeness [%] | 99.9 | 97.2 | 100 |
| Redundancy | 6.3 | 5.9 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 18 % PEG 8000, 0.2 M calcium acetate, 0.1 M sodium cacodylate pH 6.5 |
