4PLO
Crystal Structure of chicken Netrin-1 (LN-LE3) in complex with mouse DCC (FN4-5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-01 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.478, 72.939, 285.159 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.750 - 2.901 |
R-factor | 0.2291 |
Rwork | 0.224 |
R-free | 0.28760 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.414 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.950 |
High resolution limit [Å] | 2.900 | 7.860 | 2.900 |
Rmerge | 0.113 | 0.038 | 0.882 |
Total number of observations | 93328 | ||
Number of reflections | 22875 | ||
<I/σ(I)> | 5.8 | ||
Completeness [%] | 96.1 | 95.9 | 85.5 |
Redundancy | 4.1 | 4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.05 M Ammonium sulfate, 0.05 M BIS-TRIS pH 6.5, 30% v/v Pentaerythritol ethoxylate |