4PLL
Structure of the chromodaomain of MRG2 in complex with H3K36me3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 61 |
Unit cell lengths | 109.157, 109.157, 29.977 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.575 - 2.600 |
R-factor | 0.2437 |
Rwork | 0.240 |
R-free | 0.27930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.330 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.094 | 0.480 |
Number of reflections | 6489 | |
<I/σ(I)> | 31 | 2.4 |
Completeness [%] | 99.1 | 97.4 |
Redundancy | 6.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 290 | 0.1M MES pH 6.5, 30% polyethylene glycol monomethyl ether 5000, 0.2M Ammonium sulfate |