4PKN

Crystal structure of the football-shaped GroEL-GroES2-(ADPBeFx)14 complex containing substrate Rubisco

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-06
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	171.952, 173.653, 411.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	122.185 - 3.660
R-factor	0.1889
Rwork	0.188
R-free	0.24100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.207
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	122.185	3.860
High resolution limit [Å]	3.660	3.660
Rmerge		0.655
Number of reflections	135754
<I/σ(I)>	6.9	2.6
Completeness [%]	99.7	99.8
Redundancy	6.1	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10 % PEG 550 mme (v/v), 0.1 M acetic acid-KCl buffer