4PKI
Complex of ATP-actin With the C-terminal Actin-Binding Domain of Tropomodulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-22 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0781 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.393, 81.211, 170.708 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.300 |
R-factor | 0.1697 |
Rwork | 0.168 |
R-free | 0.20050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1io0 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.431 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.063 | 0.570 |
Number of reflections | 43679 | |
<I/σ(I)> | 32.8 | 3.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.8 | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.25 M sodium chloride, 12% w/v PEG3350 |