4PKG

Complex of ATP-actin With the N-terminal Actin-Binding Domain of Tropomodulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-10
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	69.471, 75.866, 136.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.800
R-factor	0.1572
Rwork	0.156
R-free	0.18700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1eqy
RMSD bond length	0.011
RMSD bond angle	1.295
Data scaling software	HKL-2000
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.910
High resolution limit [Å]	1.800	1.800
Rmerge	0.050	0.576
Number of reflections	67450
<I/σ(I)>	43.7	2.5
Completeness [%]	99.8	99.4
Redundancy	13.4	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	289	0.18 M sodium fluoride, 11% w/v PEG3350, 1% v/v PEG1000, 1% v/v PEG400