4PK9
The Crystal Structure of Native Patatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 93.15 |
| Detector technology | CCD |
| Collection date | 2008-07-31 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 53.316, 53.316, 244.947 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.320 - 1.960 |
| R-factor | 0.1943 |
| Rwork | 0.193 |
| R-free | 0.22360 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oxw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.990 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.885 | 2.030 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 24922 | |
| <I/σ(I)> | 20 | 5 |
| Completeness [%] | 93.2 | 76.4 |
| Redundancy | 5.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294.15 | 0.1 M sodium acetate, 49% v/v PEG400, 225 mM cesium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |
