4PK0

CRYSTAL STRUCTURE OF T4 LYSOZYME-PEPTIDE IN COMPLEX WITH TEICOPLANIN-A2-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-03
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97923
Spacegroup name	I 2 2 2
Unit cell lengths	55.923, 63.233, 138.615
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.1915
Rwork	0.189
R-free	0.23721
RMSD bond length	0.009
RMSD bond angle	1.474
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.146	1.000
Number of reflections	11381
<I/σ(I)>	11.4	2.1
Completeness [%]	99.8	100
Redundancy	5.9	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	19% PEG 3350, 0.2 M sodium potassium phosphate