4PJY
Azide bound Cysteine Dioxygenase at pH 6.2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 57.600, 57.600, 122.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.294 - 1.501 |
R-factor | 0.1779 |
Rwork | 0.174 |
R-free | 0.21150 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.283 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.000 | 1.580 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 33827 | |
<I/σ(I)> | 8.6 | 0.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.8 | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | Purified enzyme was concentrated to ~8 mg/mL and then added into a crystallization screen containing 0.1 M tri-sodium citrate pH=5.6, 24-34% PEG 4K, and 0.1-0.25 M ammonium acetate. 1.5L of protein solution was added to each well and mixed with an equivalent volume of reservoir solution, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |