4PJQ
Crystal structure of designed cPPR-polyG protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | F 2 3 |
Unit cell lengths | 207.543, 207.543, 207.543 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.614 - 3.353 |
R-factor | 0.1956 |
Rwork | 0.190 |
R-free | 0.24750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pjs |
RMSD bond length | 0.003 |
RMSD bond angle | 0.685 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.614 | 3.620 |
High resolution limit [Å] | 3.350 | 3.350 |
Rmerge | 0.057 | 1.011 |
Number of reflections | 20728 | |
<I/σ(I)> | 17.8 | 2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 6.9 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 291.15 | 0.1M BisTris pH 6.7, 22% PEG3350 |