4PJN
Myosin VI motor domain in the Pi release state, space group P212121 - shortly soaked with PO4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.320, 95.750, 103.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.280 - 2.000 |
| R-factor | 0.1722 |
| Rwork | 0.170 |
| R-free | 0.20720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.125 | 0.880 |
| Number of reflections | 61572 | |
| <I/σ(I)> | 13.64 | 1.94 |
| Completeness [%] | 99.4 | 96.8 |
| Redundancy | 8.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 7% PEG8K, 50mM Tris pH8,5, 1mM TCEP, 3% Glyc?rol, then soaked with 30% Glycerol and finaly soaked few secondes with 100mM NaH2PO4 pH 7.5 |
