4PJN
Myosin VI motor domain in the Pi release state, space group P212121 - shortly soaked with PO4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.320, 95.750, 103.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.280 - 2.000 |
R-factor | 0.1722 |
Rwork | 0.170 |
R-free | 0.20720 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.125 | 0.880 |
Number of reflections | 61572 | |
<I/σ(I)> | 13.64 | 1.94 |
Completeness [%] | 99.4 | 96.8 |
Redundancy | 8.2 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 7% PEG8K, 50mM Tris pH8,5, 1mM TCEP, 3% Glyc?rol, then soaked with 30% Glycerol and finaly soaked few secondes with 100mM NaH2PO4 pH 7.5 |