4PJL

Myosin VI motor domain A458E mutant in the Pi release state, space group P212121 -

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-03-11
Detector	PSI PILATUS 6M
Wavelength(s)	0.953730
Spacegroup name	P 21 21 21
Unit cell lengths	91.180, 95.320, 104.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.000 - 2.100
R-factor	0.1681
Rwork	0.166
R-free	0.20380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pfo
RMSD bond length	0.010
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.11.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.150
High resolution limit [Å]	2.100	2.100
Rmerge	0.087	0.651
Number of reflections	53412
<I/σ(I)>	13.34	2.28
Completeness [%]	99.3	93.8
Redundancy	4.82	4.84

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	7% PEG8k, 50mM Tris pH 7,5, 1mM TCEP, 10% Glycerol