4PJL
Myosin VI motor domain A458E mutant in the Pi release state, space group P212121 -
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.953730 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 91.180, 95.320, 104.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.000 - 2.100 |
R-factor | 0.1681 |
Rwork | 0.166 |
R-free | 0.20380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pfo |
RMSD bond length | 0.010 |
RMSD bond angle | 1.010 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.150 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.087 | 0.651 |
Number of reflections | 53412 | |
<I/σ(I)> | 13.34 | 2.28 |
Completeness [%] | 99.3 | 93.8 |
Redundancy | 4.82 | 4.84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 7% PEG8k, 50mM Tris pH 7,5, 1mM TCEP, 10% Glycerol |