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4PJL

Myosin VI motor domain A458E mutant in the Pi release state, space group P212121 -

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID29
Synchrotron siteESRF
BeamlineID29
Temperature [K]100
Detector technologyPIXEL
Collection date2011-03-11
DetectorPSI PILATUS 6M
Wavelength(s)0.953730
Spacegroup nameP 21 21 21
Unit cell lengths91.180, 95.320, 104.020
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution42.000 - 2.100
R-factor0.1681
Rwork0.166
R-free0.20380
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4pfo
RMSD bond length0.010
RMSD bond angle1.010
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwareBUSTER (2.11.5)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.150
High resolution limit [Å]2.1002.100
Rmerge0.0870.651
Number of reflections53412
<I/σ(I)>13.342.28
Completeness [%]99.393.8
Redundancy4.824.84
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.52777% PEG8k, 50mM Tris pH 7,5, 1mM TCEP, 10% Glycerol

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