4PJJ
MYOSIN VI (MD-INSERT2-CAM, DELTA-INSERT1) post-rigor state - long soaking with PO4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-04-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9801 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 73.010, 109.240, 180.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.740 - 2.400 |
R-factor | 0.1741 |
Rwork | 0.172 |
R-free | 0.20670 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vas |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.110 | 1.076 |
Number of reflections | 57130 | |
<I/σ(I)> | 14.23 | 2.31 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 9.57 | 9.69 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 9% PEG8k, 50mM Hepes pH 7,5, 1mM TCEP, 3% EG, 3% MPD |