4PIC
YwlE arginine phosphatase from Geobacillus stearothermophilus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.434, 75.753, 47.717 |
| Unit cell angles | 90.00, 92.37, 90.00 |
Refinement procedure
| Resolution | 17.994 - 1.400 |
| R-factor | 0.1804 |
| Rwork | 0.179 |
| R-free | 0.20010 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.049 |
| Data scaling software | SCALA (3.3.9) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 17.994 | 17.994 | 1.480 |
| High resolution limit [Å] | 1.400 | 4.430 | 1.400 |
| Rmerge | 0.032 | 0.336 | |
| Rmeas | 0.084 | ||
| Rpim | 0.033 | 0.022 | 0.163 |
| Total number of observations | 361103 | 12092 | 52402 |
| Number of reflections | 59452 | ||
| <I/σ(I)> | 14.6 | 32.5 | 4.3 |
| Completeness [%] | 95.3 | 96.5 | 93.7 |
| Redundancy | 6.1 | 6.2 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 298 | 2 M ammonium sulfate and 100 mM Tris/HCl |
