4PIB
Crystal Structure of Uncharacterized Conserved Protein PixA from Burkholderia thailandensis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97901 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 79.806, 84.868, 249.693 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.111 - 2.000 |
| R-factor | 0.183 |
| Rwork | 0.181 |
| R-free | 0.22400 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.219 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: dev_1621)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 114270 | |
| <I/σ(I)> | 8.2 | 3.1 |
| Completeness [%] | 99.7 | 99.6 |
| Redundancy | 6.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M calcium chloride, 0.1 M HEPES pH 8.5, 20 %(w/v) PEG4000 |
