4PHY
Functional conservation despite structural divergence in ligand-responsive RNA switches
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 173 |
Detector technology | IMAGE PLATE |
Collection date | 2013-09-06 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.54 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 79.611, 79.611, 100.940 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.375 - 3.100 |
R-factor | 0.2173 |
Rwork | 0.215 |
R-free | 0.26790 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 1.070 |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.900 | 3.210 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 3167 | |
<I/σ(I)> | 22.28 | 2.5 |
Completeness [%] | 88.2 | 56.4 |
Redundancy | 28 | 15.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | KCl, Magnesium Acetate, PEG 8K |