4PHS

Selenomethionine substituted structure of domain of unknown function 1792 (DUF1792)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-30
Detector	MAR CCD 165 mm
Wavelength(s)	0.97142, 0.97907, 0.97877
Spacegroup name	C 1 2 1
Unit cell lengths	71.069, 44.596, 78.752
Unit cell angles	90.00, 110.51, 90.00

Refinement procedure

Resolution	29.130 - 1.540
R-factor	0.205
Rwork	0.202
R-free	0.24800
RMSD bond length	0.007
RMSD bond angle	1.271
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.540
Number of reflections	34221
<I/σ(I)>	33.1
Completeness [%]	99.6
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris, PEG 1500, NaAcetate