4PHP

Structure of human DNA polymerase beta complexed with T in the template base paired with incoming non-hydrolyzable GTP and MANGANESE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-05-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	50.701, 79.327, 55.474
Unit cell angles	90.00, 107.58, 90.00

Refinement procedure

Resolution	19.833 - 2.600
R-factor	0.185
Rwork	0.181
R-free	0.26160
RMSD bond length	0.004
RMSD bond angle	0.997
Data reduction software	DENZO
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Number of reflections	12915
<I/σ(I)>	9.3
Completeness [%]	99.9
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	14%?23% PEG3400, and 350 mM sodium acetate in 50 mM imidazole (pH 7.5)