4PHF

Crystal structure of Ypt7 covalently modified with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	49.310, 54.900, 60.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.650 - 1.950
R-factor	0.19882
Rwork	0.196
R-free	0.24492
RMSD bond length	0.019
RMSD bond angle	1.859
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.650	2.050
High resolution limit [Å]	1.950	1.950
Number of reflections	12456
<I/σ(I)>	12.41
Completeness [%]	100.0
Redundancy	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.5 M NH4NO3, 16% (w/v) PEG3350