4PH3
N-terminal domain of the capsid protein from bovine leukaemia virus (with no beta-hairpin)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-05-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.613, 66.242, 81.355 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.370 - 2.440 |
| R-factor | 0.2092 |
| Rwork | 0.206 |
| R-free | 0.24540 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data scaling software | XDS |
| Refinement software | BUSTER-TNT (BUSTER 2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.355 | 2.447 |
| High resolution limit [Å] | 2.439 | 2.439 |
| Rmerge | 0.080 | 0.280 |
| Number of reflections | 13762 | |
| <I/σ(I)> | 39.7 | 11.1 |
| Completeness [%] | 100.0 | 98.4 |
| Redundancy | 14.2 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 100 mM MES pH 5.0, 1.6 M ammonium sulphate |
