4PH2

mature N-terminal domain of capsid protein from bovine leukemia virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-11-14
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	40.670, 56.040, 103.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.740 - 1.440
R-factor	0.164
Rwork	0.163
R-free	0.18580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ph3
RMSD bond length	0.006
RMSD bond angle	1.052
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.530		1.540
High resolution limit [Å]	1.440	15.000	1.440
Rmerge	0.057	0.028	0.225
Rmeas	0.065	0.033	0.255
Total number of observations	174086
Number of reflections	40094	40	7138
<I/σ(I)>	16.31	24.48	6.82
Completeness [%]	92.2	72.7	91.7
Redundancy	4.3		4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	0.2M AmAc, 1M Ammonium sulphate