4PH0
capsid protein from bovine leukemia virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 65 |
| Unit cell lengths | 94.280, 94.280, 257.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.140 - 2.750 |
| R-factor | 0.1871 |
| Rwork | 0.180 |
| R-free | 0.22130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.362 |
| Data scaling software | XDS |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.140 | 2.920 | |
| High resolution limit [Å] | 2.750 | 8.140 | 2.750 |
| Rmerge | 0.067 | 0.026 | 1.015 |
| Rmeas | 0.070 | 0.028 | 1.068 |
| Total number of observations | 351378 | ||
| Number of reflections | 33567 | 1320 | 5374 |
| <I/σ(I)> | 22.42 | 77.49 | 2.05 |
| Completeness [%] | 99.9 | 98.7 | 99.5 |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20mM Tris.HCl, 130mM NaCl, 25% PEG 4000 |
