4PGU
Crystal structure of YetJ from Bacillus Subtilis at pH 7 by soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 62.207, 62.207, 293.474 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.689 - 3.401 |
| R-factor | 0.2752 |
| Rwork | 0.273 |
| R-free | 0.31290 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.696 |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 5281 |
| <I/σ(I)> | 10.2 |
| Completeness [%] | 99.9 |
| Redundancy | 19.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 30% PEG 600, 100 mM CaCl2, 100 mM Tris-HCl, pH 8.0 |
